Device

Part:BBa_K258103

Designed by: Hikmet Geckil, Fulbright Postdoctoral Researcher   Group: iGEM09_METU-Gene   (2009-10-21)


Vhb protein

The gene coding for the Vitreoscilla hemoglobin (VHb) molecule has been cloned and functionally expressed in Escherichia coli. By using a plasmid-encoded gene as well as single-copy integrants, the oxygen-dependent VHb gene (VHb) promoter was shown to be functional in E. coli. The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation). We wanted to measure the activity of VHb gene; therefore, the VHb promoter. The VHb promoter becomes active when the surrounding oxygen amount lowers to 2%. We wanted to see whether this promoter will effectively work with our E. Coli cells.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 388
    Illegal AgeI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None